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HEALTHCARE PROFESSIONALS
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SCIENTIFIC BRIEFING FOR
HEALTHCARE PROFESSIONALS AND BIOMEDICAL INVESTIGATORS
Diindolylmethane (DIM)
Immune Modulating Properties
Stimulation of Interferon- γ
sensitivity by increasing IFN-γ
receptors
Stimulation of Interferon-γ,
G-CSF, IL-12 & IL-6
production
Synergy with Interferon-γ
in expression of the MHC-I complex
Selection of Data from the Reference Section
DIM In-Vivo Affect on Key Immune
Modulating Cytokines
In this study DIM was
administered orally at 30mg/kg of body weight.
DIM In-Vivo Anti-Viral Activity
Through Immune Modulation
(Reference: Xue L, Pestka J, Maoxiang L,
Firestone GL, Bjeldanes LF, 3,3′-Diindolylmethane stimulates murine
immune function in vitro and in vivo, Journal of Nutritional
Biochemistry,
published online, 8-20-07.)
DIM Stimulates Transcript Expression of Interferon-γ
and Related Genes in Human Breast Cancer Cells
Activation and Potentiation of Interferon-γ by DIM:
Stimulation of Interferon-γ Receptor 1 Production
Potentiation of Interferon-γ Mediated Cell Cycle Arrest by DIM in Human Breast Cancer Cells
DIM Enhances Interferon-γ Induced Expression of MHC-I Complex in Human Breast Cancer Cells
(Please Note Section D of this figure.
DIM may be used as an adjuvant
to IFNγ treatment models because of this unique synergistic effect on the MHC-I Complex.
This synergy is currently under investigation for HIV, HPV, Hepatitis
and multiple forms of cancer.)
Fig 8. The expression level of MHC-I complex on the MCF-7 cell
surface. A, cells were treated with different concentrations of IFN γ
for 16 h. B and C, cells
were pretreated with DMSO (control) or 30
µM DIM for 48 h, followed by 0.1 ng/ml of
IFNγ
(B) or 10 ng/ml of IFNγ
(C) for another 16 h. One
million cells were incubated in 90
µl of
PBS with 10
µl of
FITC-conjugated anti-HLA-ABC mouse monoclonal antibody. The fluorescence
intensity was measured by flow cytometry using a Coulter Elite instrument and
analyzed by WinMDI 2.8 software provided by Duke University. D, the expression
of HLA class I complex was calculated as %PC (open bars) and MFV (cross-hatched
bars).
(Reference: Riby
JE, Xue L, Chatterji U, Bjeldanes EL, Firestone GL, Bjeldanes LF,
Activation and Potentiation of Interferon-(gamma) Signaling by
3,3'-Diindolylmethane in MCF-7 Breast Cancer Cells, Molecular
Pharmacology, Nov. 2, 2005)
DIM and Taxol Synergize to Promote Apoptosis
(Reference: K. McGuire, N. Ngoubilly, M. Neavyn,
S. Lanza-Jacoby. 3,3′-Diindolylmethane and Paclitaxel Act Synergistically
to Promote Apoptosis in HER2/Neu Human Breast Cancer Cells. Journal of
Surgical Research, 2006 May 15;132(2):208-13. Department of Surgery,
Jefferson Medical College, Thomas Jefferson University, Philadelphia,
Pennsylvania 19107)
In-Vivo Animal Study: DIM Inhibits DMBA-induced
Mammary Tumor Growth
(Reference: Chen I, McDougal A, Wang F, Safe S,
Carcinogenesis, vol. 19, no.9. pp.1631. 1998.)
In-Vivo Animal Study: DIM Inhibits Growth
of Human Mammary Tumor Cells in Athymic Mice
(Reference: Chang
X., Tou J.C., Hong C., Kim H.A., Riby J.E., Firestone G.L., Bjeldanes L.F.,
3,3'-Diindolylmethane inhibits angiogenesis and the growth of
transplantable human breast carcinoma in athymic mice, Carcinogenesis,
Janurary 2005, pp.771-778.)
Additional information about
DIM is available at the
Diindolylmethane Information Resource Center (www.diindolylmethane.org).
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